产品编号:I-395-2
产品描述:Recombinant Human IL-12
反应种属:
实验方法:
标记:
规格:2ug
供应商:Leinco
价格(RMB):1485.00
订购:
说明书:
Recombinant Human Interleukin-12 (IL-12)
Purified No Carrier Protein
(NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), p35, CLMF2, IL-12B, NKSF2)
Prod. No.: I-395
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 2 µg
Storage: -20°C to -70°C
Background:
Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells1 , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.2 IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.3
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-12 is Mr 34.7 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 μg of bovine serum albumin per 1 μg of cytokine with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The monomeric human IL-12 p40 monomer exhibits no IL-12 antagonist activity. rhIL-12 p40 is intended for use as a control for anti-human IL-12 p40 antibodies.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
iwelkkdv yvveldwypd apgemvvltc dtpeedgitw tldqssevlg sgktltiqvk efgdagqytc hkggevlshs llllhkkedg iwstdilkdq kepknktflr ceaknysgrf tcwwlttist dltfsvkssr gssdpqgvtc gaatlsaerv rgdnkeyeys vecqedsacp aaeeslpiev mvdavhklky enytssffir diikpdppkn lqlkplknsr qvevsweypd twstphsyfs ltfcvqvqgk skrekkdrvf tdktsatvic rknasisvra qdryysssws ewasvpcs
References
1. Kapsenberg ML et al. (1997) J. Immunol. 159: 28
2. Jerome Ritz. et al. Blood 95: 3190
3. Barnes PF. et al. J. Clin. Invest. 93: 1733
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