产品编号:I-379-50
产品描述:Recombinant Human IL-9
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):13650.00
订购:
说明书:
Recombinant Human Interleukin-9 (IL-9)
Purified No Carrier Protein
(P40 Cytokine, T-Cell Growth Factor P40, HP40, P40 )
Prod. No.: I-379
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin-9 (IL-9), also known as P40, is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes (1). This cytokine stimulates cell proliferation and prevents apoptosis. IL-9 functions through the IL-9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes (2). It enhances the proliferation of T lymphocytes, mast cells, erthroid precursor cells and megakaryoblastic leukemia cell lines. Th2 cells have historically been thought to be the source of IL-9. However, recent data suggest the existence of a Th2-related cell type that is characterized by the secretion of IL-9 and IL-10 (3). These so-called Th9 cells can differentiate from Th2 cells in the presence of TGF-β, or they can differentiate from a naïve CD4 cell with a combination of IL-4 and TGF-β. IL-9 plays a role in the complex pathogenesis of bronchial hyperresponsiveness as a risk factor for asthma (4). Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkins disease (5).
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-9 is Mr 14.1 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 16-25 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-9 was determined by its ability to stimulate cell proliferation in the factor-dependent human megakaryocytic leukemic cell line, M07e. (Avanzi, G. et al., 1988, British Journal of Haematology 69:359 - 366). The expected ED50 for this effect is typically 0.5 - 1.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
qg cptlagildi nflinkmqed paskchcsan vtsclclgip sdnctrpcfs erlsqmtntt mqtryplifs rvkksvevlk nnkcpyfsce qpcnqttagn altflkslle ifqkekmrgm rgki
References
1. Renauld, J. C. et al. (1995) J. Immunol. 154:5061
2. Yang, Y. C. et al. (1995) J. Biol. Chem. 270:20497
3. Veldhoen, M. et al. (2008) Nature Immunol. 9:1341
4. Levitt, R. C. et al. (1997) Proc. Natl. Acad. Sci. (USA) 94:13175
5. Gruss, H. J. et al. (1992) Cancer Res. 52:1026
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