产品编号:I-190-50
产品描述:Recombinant Human IL-8
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):6900.00
订购:
说明书:
Recombinant Human Interleukin-8 (IL-8)
Purified No Carrier Protein
(CXCL8, MDNCE, Neutrophil Activating Factor (NAF), NAP-1 Fractalkine Mucin-Like Stalk, 3-10C, AMCF-I, GCP-1, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, AP1, SCYB8, TSG-1, B-ENAP)
Prod. No.: I-190
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin 8 (IL-8), also known as CXCL8, is a member of the CXC chemokine family. It is secreted by a variety of cell types including monocytes, macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL-1, TNF, LPS) (1). It is also synthesized by endothelial cells, which store IL-8 in vesicles. IL-8 functions as a chemoattractant for neutrophils, basophils, and T cells, but not monocytes (2). It also plays a vital role in tumorigenesis, progression and metastasis, and accordingly the expression levels of IL-8 correlate with disease progression in several human carcinomas (3). IL-8 has the N-terminal tripeptide ELR motif, which is known to mediate IL-8 receptor binding and subsequent signal transduction, such as the TRAF6-NF-κB pathway. It can also signal through the CXCR1 and CXCR2 receptors. IL-8 is believed to play a role in the pathogenesis of cystic fibrosis (4).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-8 is Mr 8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in deionized water.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-8 was determined by its induction of myeloperoxidase release from human neutrophils (Schröeder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of hCXCR-2 transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 0.15 - 0.3 μg/ml, or 0.5 - 2.5 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
sak elrcqcikty skpfhpkfik elrviesgph canteiivkl sdgrelcldp kenwvqrvve kflkraens
References
1. Baggiolini, M. et al. (1992) FEBS Lett. 307:97
2. Köhidai, L. et al. (1998) Cytokine 10:481
3. Yuan, A. et al. (2006) Front. Biosci. 10:853
4. Tabary, O. et al. (1998) Amer. J. Pathol. 153:921
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