产品编号:I-189-1
产品描述:Recombinant Human IL-7
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Interleukin-7 (IL-7)
Purified No Carrier Protein
(Lymphopoietin 1, LP-1, Pre-B Cell Factor)
Prod. No.: I-189
Source E. coli
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin 7 (IL-7), also known as Lymphopoietin 1, is a hematopoietic growth factor which affects primarily early B and T cells (1). It stimulates the differentiation of multipotent hematopoietic stem cells into lymphoid progenitor cells. IL-7 is important for proliferation during certain stages of B cell maturation, T and NK cell survival, development and homeostasis. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2 (2). In myeloid lineage cells, IL-7 up-regulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes and macrophages. IL-7 has significant clinical and biomedical potential, such as cancer therapy and HIV infections (3). Human and murine IL-7 is cross-species reactive.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-7 is Mr 17 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-7 was Measured as its T cell growth factor activity in a cell proliferation assay using PHA activated human peripheral blood lymphocytes (Yokota, T. et al., 1986, Proc. Nat. Acad. Sci. USA 88:5894). The expected ED50 for this effect is typically 0.2 - 0.5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mdcdie gkdgkqyesv lmvsidqlld smkeigsncl nnefnffkrh icdankegmf lfraarklrq flkmnstgdf dlhllkvseg ttillnctgq vkgrkpaalg eaqptkslee nkslkeqkkl ndlcflkrll qeiktcwnki lmgtkeh
References
1. Namen, A. E. et al. (1990) Int. J. Cell Cloning 1:168
2. Reed, S. G. et al. (1989) J. Exp. Med. 169:707
3. Prakash, S. et al. (2008) Appl. of Biochem. Biotechnol. 151:93
Related Products
Anti-Mouse IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-259)
Anti-Human IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-151)
Anti-Mouse IL-7 (Antigen Aff. Pur.) (Leinco Prod. No.: I-166)
Anti-Human IL-7 (Leinco Prod. No.: I-175)
Recombinant Rat IL-7 (Leinco Prod. No.: I-192)
Recombinant Mouse IL-7 (Leinco Prod. No.: I-209)
Human IL-7 ELISA Development Kit (Leinco Prod. No.: I-826)
Anti-Human IL-7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-258)
