产品编号:I-537-50
产品描述:Recombinant Mouse IL-1 R4
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Mouse IL-1 R4
Purified No Carrier Protein
(ST2, T1, St2, DER4, Ly84, ST2L, Fit-1, T1/ST2, St2-rs1, Il1rl1)
Prod. No.: I-537
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C
Description
Background:
ST2, also known as IL-1 R4 and T1, is a transmembrane glycoprotein. It belongs to the Interleukin-1 receptor family and is involved in Th2 immune responses (1). ST2 is expressed on mast cells, Th2 cells, macrophages as well as on stressed cardiac myocytes and airway epithelia (2-7). ST2 binds to IL-33, a pro-inflammatory IL-1 family cytokine which induces helper T cells, mast cells, eosinophils, and basophils to produce type 2 cytokines (8-9). IL-33 binding induces the association of ST2 with IL-1 R1 and in macrophages, ST2 interferes with signaling from IL-1 R1 and TLR4 (1, 4). Soluble ST2 can also bind IL-33 to function as a decoy receptor that blocks the ability of IL-33 to signal through transmembrane ST2 (10).
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse IL-1 R4 is Mr 63 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 73 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IL-1 R4 was determined by its ability to bind rmIL-33 in a functional ELISA type binding assay. The estimated KD is < 50 nM.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
skss wglenealiv rcpqrgrsty pvewyysdtn esiptqkrnr ifvsrdrlkf lparvedsgi yacvirspnl nktgylnvti hkkppscnip dylmystvrg sdknfkitcp tidlynwtap vqwfknckal qeprfrahrs ylfidnvthd degdytcqft haengtnyiv tatrsftvee kgfsmfpvit nppynhtmev eigkpasiac sacfgkgshf ladvlwqink tvvgnfgear iqeeegrnes ssndmdclts vlritgvtek dlsleydcla lnlhgmirht irlrrkqpid hrsiyyivag csiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgk
References
1. Barksby, H. E. et al. (2007) Clin. Exp. Immunol. 149:217
2. Schmitz, J. et al. (2005) Immunity 23:479
3. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979
4. Brint, E. K. et al. (2004) Nat. Immunol. 5:373
5. Weinberg, E. O. et al. (2002) Circulation 106:2961
6. Xu, D. et al. (1998) J. Exp. Med. 187:787
7. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930
8. Kato, A. et al. (2007) Curr. Opin. Immunol. 19:711
9. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051
10. Palmer, G. et al. (2008) Cytokine 42:358
