产品编号:I-374-1
产品描述:Recombinant Human IGFBP-5
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Insulin-Like Growth Factor Binding Protein 5 (IGFBP-5)
Purified No Carrier Protein
(BP5)
Prod. No.: I-374
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Insulin-like growth factor binding protein 5, also known as IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. It is expressed highly in the kidney. IGFBP-5 has an autonomous role in the regulation of cell fate in development and in tumourigenesis.1
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IGFBP-5 is Mr 28 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 34 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGFBP-5 was determined by by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is typically 0.5 - 1.5 μg/ml in the presence of 14 ng/ml rhIGF-II.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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References
1. Jennifer M. Pell et al. (2004) J of Cell Science 117: 1737
