产品编号:I-370-25
产品描述:Recombinant Human IGFBP-1
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Insulin-Like Growth Factor-Binding Protein 1 (IGFBP-1)
Purified No Carrier Protein
(IBP-1, Placenta Protein 12 (PP12), AFBP, IGF-BP25, HIGFBP-1)
Prod. No.: I-370
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Insulin-like growth factor binding protein 1, also known as IGFBP1, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is particularly important in female reproductive physiology, where it is involved with other factors in a complex system which regulates menstrual cycles, puberty, ovulation, decidualization, implantation and fetal growth. There is evidence for a pathophysiological role for IGFBP-1 in pre-eclampsia, intrauterine growth restriction, polycystic ovarian syndrome and trophoblast and endometrial neoplasms.1
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IGFBP-1 is Mr 25 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 32 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGFBP-1 was determined by by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is typically 1.0 - 4.0 μg/ml in the presence of 6 ng/ml rhIGF-I.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
apwqc apcsaeklal cppvsascse vtrsagcgcc pmcalplgaa cgvatarcar glscralpge qqplhaltrg qgacvqesda saphaaeags pespesteit eeelldnfhl mapseedhsi lwdaistydg skalhvtnik kwkepcriel yrvveslaka qetsgeeisk fylpncnkng fyhsrqcets mdgeaglcwc vypwngkrip gspeirgdpn cqiyfnvqn
References
1. J. P. Miell et al. (2000) Human Reproduction 6: 495
Related Products
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Anti-Human IGFBP-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-746)
Anti-Human IGFBP-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-805)
