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Recombinant Human IGF-BP7

产品编号:I-335-25

产品描述:Recombinant Human IGF-BP7

反应种属:

实验方法:

标记:

规格:25ug

供应商:Leinco

价格(RMB):4725.00

订购:订购

说明书:

Recombinant Human Insulin-Like Growth Factor-Binding Protein 7 (IGF-BP7)
Purified No Carrier Protein
(IBP-7, Mac25, IGF Binding Protein Related Protein-1 [IGFBPrP1], FSTL2, IGFBP-7, IGFBP-7v, PSF)
Prod. No.: I-335
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C

Description
Background:

Insulin-like growth factor binding protein 7 (IGFBP7), also known as Mac25 and IGFBP-rp1, is a secretory protein. IGFBP7 is expressed in a wide range of normal human tissues with very high levels in HEVs of blood vessels in the secondary lymphoid tissues. IGFBP7 suppresses growth and colony formation of prostate and breast cancer cell lines through an IGF independent mechanism, which causes a delay in the G1 phase of the cell cycle and increased apoptosis. (1-2). The expression of IGFBP7 is upregulated in senescing epithelial cells and by retinoic acid (3). It plays a role in skeletal myogenesis by binding to IGF in a manner that inhibits IGF induced differentiation of skeletal myoblasts, without affecting IGF induced proliferation. IGFBP7 also interacts with heparin sulfate proteoglycans, type IV collagen and specific chemokines. It has also been implicated in angiogenisis. 

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IGF-BP7 is Mr 27.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 36 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IGF-BP7 was determined by by its ability to bind rh6Ckine/CCL21. Immobilized rhIGFBP-rp1 at 1 μg/ml (100 μl/well) can bind rh6Ckine/CCL21 with a linear range of 0.08 - 5 ng/ml in a functional ELISA. (Nagakubo, D. et al., 2003, J.Immunol. 171:553).
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

d tcgpcepasc pplpplgcll getrdacgcc pmcargegep cggggagrgy capgmecvks rkrrkgkaga aaggpgvsgv cvcksrypvc gsdgttypsg cqlraasqra esrgekaitq vskgtceqgp sivtppkdiw nvtgaqvyls cevigiptpv liwnkvkrgh ygvqrtellp gdrdnlaiqt rggpekhevt gwvlvsplsk edageyecha snsqgqasas akitvvdalh eipvkkgega elhhhhhhhh hh

References
1. Tamura, K. et al. (2008) Reprod. Biol. Endocrinol. 6:54
2. Lai, M. et al. (2007) Cancer Biol. Ther. 6:354
3. Swisshelm, K. et al. (1995) Proc. Nat. Acad. Sci. (USA) 92:4474