产品编号:I-538-1
产品描述:Recombinant Mouse IFN-γ R1
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Interferon-Gamma Receptor 1 (IFN-γ R1)
Purified No Carrier Protein
(CD119, IFN-γR, IFN-γRα, IFN-α R1, CDw119)
Prod. No.: I-538
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C
Description
Background:
IFN-γ receptor ligand-binding chain (IFNγR1) or IFN-γ R α chain, also known CDw119, is member of the class II cytokine receptor family. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.1,2,3
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse IFN-γ R1 is Mr 52 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 70-90 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse IFN-γ R1 was determined by its ability to inhibit rmIFN-γ mediated protection of mouse L929 cells viral lysis. The expected ED50 is typically 0.4 - 2 μg/mL in the presence of 1 ng/mL of rmIFN-γ.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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References
1. Calderon, J. et al. (1988) PNAS 85: 4837
2. A. Garcia-Sastre. et al. (2002) Int. Rev. Immunol. 21: 305
3. E. Fish. et al. (2002) J. Interferon Cytokine Res. 22: 835
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Anti-Human IFN-γ R1-Fluorescein (FITC) (Leinco Prod. No.: I-824)
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