产品编号:I-376-1
产品描述:Recombinant Human IL-29
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Interleukin-29 (IL-29)
Purified No Carrier Protein
(IFN-λ1, IFNL1 (IFN-Lambda1), λ1)
Prod. No.: I-376
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin-29 (IL-29), also known as IFN-λ1, is a cytokine distantly related to type I interferons and the IL-10 family. It exerts its biological effects through a receptor that also binds IL-28A and IL-28B (1). This receptor is a heterodimeric class II cytokine receptor that consists of IL-10RB and IL-28RA. IL-29 activities overlap those of type I IFNs and include anti-viral activity and up-regulation of MHC class I antigen expression (2). The expression of IL-29 is induced by virus infection or double-stranded RNA. Upon binding to the receptor, it also induces JAK kinase activation and phosphorylation of STAT and STAT2 (3). IL-28 and IL-29 have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells (4).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-29 is Mr 21.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 26-35 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IFN-Lambda1 was determined in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis (Sheppard, P. et al., 2003, Nature Immunol. 4:63). The expected ED50 for this effect is typically 1 - 5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
g pvptskpttt gkgchigrfk slspqelasf kkardalees lklknwscss pvfpgnwdlr llqvrerpva leaelaltlk vleaaagpal edvldqplht lhhilsqlqa ciqpqptagp rprgrlhhwl hrlqeapkke sagcleasvt fnlfrlltrd lkyvadgnlc lrtsthpest hhhhhhhhhh
References
1. Zitzmann, K. et al. (2006) Biochem. Biophys. Research Commun. 344:1334
2. Kotenko, S. V. et al. (2003) Nat. Immunol. 4:69
3. Maher, S. G. et al. (2008) Cancer Biol. Ther. 7:1109
4. Mennechet, F. et al. (2006) Immunobiology 107:4417
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