产品编号:I-377-25
产品描述:Recombinant Human IL-28A
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Interleukin-28A (IL-28A)
Purified No Carrier Protein
(IFNL2 (IFN-Lambda-2 Protein), IFNλ2)
Prod. No.: I-377
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Interleukin-28A (IL-28A), also known as IFN-λ2, is a cytokine distantly related to type I interferons and the IL-10 family. It exerts its biological effects through a receptor that also binds IL-28B and IL-29 (1). This receptor is a heterodimeric class II cytokine receptor that consists of IL-10RB and IL-28RA. IL-28A activities overlap those of type I IFNs and include anti-viral activity and up-regulation of MHC class I antigen expression (2). The expression of IL-28A is induced by virus infection or double-stranded RNA. Upon binding to the receptor, it also induces JAK kinase activation and phosphorylation of STAT and STAT2 (3). IL-28A represents a potential novel approach for therapy of Th1-mediated inflammatory diseases such as T cell-mediated hepatitis (4).
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human IL-28A is Mr 20.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 24 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IFN-λ2 was determined by in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis virus (Sheppard, P. et al., 2003, Nature Immunol. 4:63). The expected ED50 for this effect is typically 1 - 5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
vpvar lhgalpdarg chiaqfksls pqelqafkra kdaleeslll kdcrchsrlf prtwdlrqlq vrerpmalea elaltlkvle atadtdpalv dvldqplhtl hhilsqfrac iqpqptagpr trgrlhhwly rlqeapkkes pgcleasvtf nlfrlltrdl ncvasgdlcv dihhhhhh
References
1. Zitzmann, K. et al. (2006) Biochem. Biophys. Research Commun. 344:1334
2. Kotenko, S. V. et al. (2003) Nat. Immunol. 4:69
3. Maher, S. G. et al. (2008) Cancer Biol. Ther. 7:1109
4. Neurath, M. F. et al. (2007) Gastroenterology 132:358
Related Products
Anti-Human IL-28A (Antigen Aff. Pur.) (Leinco Prod. No.: I-382)
Recombinant Mouse IL-28A (Leinco Prod. No.: I-378)
Anti-Human IL-28A-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-863)
