产品编号:I-223-1
产品描述:Recombinant Rat IFN-γ
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Rat Interferon-Gamma (IFN-γ)
Purified No Carrier Protein
(Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI)
Prod. No.: I-223
Source E. coli
Pkg. Size: 1 mg, 100 µg
Storage: 2°C to 8°C
Description
Background:
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Rat IFN-gamma is Mr 15.5 kDa.
State of Matter
Solution
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>97% by SDS Page and analyzed by silver stain.
Storage and Stability
This recombinant protein solution is stable for three months when stored at 2°C to 8°C.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Rat IFN-Gamma was determined in an anti-viral assay using L-929 cells infected with EMC virus (Meager, A., 1987, Lymphokines and Interferons, a Practical Approach, M.J. Clemens, Morris, A.G. and Gearing, A.J.H., eds., IRL Press, p. 129). The expected ED50 for this effect is 1 - 3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
mqgtliesl eslknyfnss smdamegksl lldiwrnwqk dgntkilesq iisfylrlfe vlkdnqaisn nisvieshli tnffsnskak kdafmsiakf evnnpqiqhk avnelirvih qlspesslrk rkrsrc
References
1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
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