产品编号:G131-50
产品描述:Recombinant Human CXCL2
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):9375.00
订购:
说明书:
Recombinant Human Chemokine (C-X-C Motif) Ligand 2 (CXCL2)
Purified No Carrier Protein
(SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), gro beta, groβ, )
Prod. No.: G131
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as MIP2-α and GROβ, is a small, inducible cytokine belonging to the CXC chemokine family. Other chemokines in this group include IL-8, GROα, GROγ, mouse KC, ENA78, GCP2 and PBP/CTAPIII/β TG/NAP2. Since it is structurally related to IL-8, CXCL2 competes with IL-8 for binding to IL-8 receptors on neutrophils and to a lesser extent, basophils. CXCL2 is involved in attraction of polymorphonuclear granulocytes to sites of infection (1). It is active against Gram-negative (Escherichia coli) and Gram-positive (Staphyllococcus aureus) bacteria. It is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells (2-4). MMP-12 cleaves CXCL2 at the ELR sequence motif, which is known to be the critical receptor binding motif leading to the loss of chemotactic activity (5).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CXCL2 is Mr 8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GRO-Beta was determined by by its ability to induce myeloperoxidase release from cytochalasin B treated neutrophils (Schröeder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of hCXCR2 transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 0.3 - 0.9 μg/ml, or 3 - 15 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
aplate lrcqclqtlq gihlkniqsv kvkspgphca qteviatlkn gqkaclnpas pmvkkiiekm lkngksn
References
1. Ulrich Beuscher, H. et al. (2004) International Immunol. 16:1675
2. Cerami, A. et al. (1989) Proc. Nat. Acad. Sci. 86:612
3. Grotendorst, G. R et al. (1990) Mol .Cell. Biol. 10:5596
4. Fukuda, S. et al. (2006) Exp. Hematol. 34:1010
5. Dean, R. A. et al. (2008) Blood 112:3455
Related Products
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G146)
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G148)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G122)
Anti-Mouse CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: M178)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G133)
Mouse CXCL2 ELISA Development Kit (Leinco Prod. No.: M1108)
Recombinant Mouse CXCL2 (Leinco Prod. No.: G632)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G136)
Human GROβ (CXCL2) ELISA Development Kit (Leinco Prod. No.: G595)
Recombinant Rat CXCL2 (Leinco Prod. No.: C1343)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: C1465)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1509)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G147)
Recombinant Mouse MIP-2 (Leinco Prod. No.: M174)
Anti-Mouse CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M195)
Anti-Rat CXCL2 (Leinco Prod. No.: C1433)
Anti-Mouse CXCL2 (Leinco Prod. No.: M1277)
