产品编号:G128-50
产品描述:Recombinant Mouse GM-CSF
反应种属:
实验方法:
标记:
规格:50ug
供应商:Leinco
价格(RMB):11925.00
订购:
说明书:
Recombinant Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF)
Purified No Carrier Protein
(CSF-2, MGI-1GM, Pluripoietin-Alpha)
Prod. No.: G128
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells.1 GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.2
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse GM-CSF is Mr 14.3 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse GM-CSF was determined by proliferation of the factor-dependent murine cell line, DA-3 (Ihle, J.N. et al., 1984, Advances in Viral Oncology, G. Klein, ed. Raven Press, New York, NY. 4:95 - 137). The expected ED50 is 10 - 60 pg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
ptrspitvtr pwkhveaike alnllddmpv tlneevevvs nefsfkkltc vqtrlkifeq glrgnftklk galnmtasyy qtycpptpet dcetqvttya dfidslktfl tdipfeckkp vqk
References
1. Micheal W. Parker et al. (2008) Cell 134: 496
2. Whitsett et al. (2002) Annual Review of Physiology 64: 775
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