产品编号:G128-1
产品描述:Recombinant Mouse GM-CSF
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF)
Purified No Carrier Protein
(CSF-2, MGI-1GM, Pluripoietin-Alpha)
Prod. No.: G128
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells.1 GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.2
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse GM-CSF is Mr 14.3 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse GM-CSF was determined by proliferation of the factor-dependent murine cell line, DA-3 (Ihle, J.N. et al., 1984, Advances in Viral Oncology, G. Klein, ed. Raven Press, New York, NY. 4:95 - 137). The expected ED50 is 10 - 60 pg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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References
1. Micheal W. Parker et al. (2008) Cell 134: 496
2. Whitsett et al. (2002) Annual Review of Physiology 64: 775
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