产品编号:G639-10
产品描述:Recombinant Rat GDNF
反应种属:
实验方法:
标记:
规格:10ug
供应商:Leinco
价格(RMB):4425.00
订购:
说明书:
Recombinant Rat Glial-Derived Neurotrophic Factor (GDNF)
Purified No Carrier Protein
(ATF-1, ATF2, HFB1-GDNF)
Prod. No.: G639
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C
Description
Background:
Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.2
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Rat GDNF is Mr 15 kDa (monomer).
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Rat GDNF was determined by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. The expected ED50 is typically 1-3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
spdkqaaalp rrernrqaaa aspensrgkg rrgqrgknrg cvltaihlnv tdlglgyetk eelifrycsg sceaaetmyd kilknlsrsr rltsdkvgqa ccrpvafddd lsflddslvy hilrkhsakr cgci
References
1. Collins F. et al. (1993)Science 260: 1130
2. Ron D et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114
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