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Recombinant Human GDNF (produced from NSO Cells)

产品编号:G715-10

产品描述:Recombinant Human GDNF (produced from NSO Cells)

反应种属:

实验方法:

标记:

规格:10ug

供应商:Leinco

价格(RMB):4425.00

订购:订购

说明书:

Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
Purified No Carrier Protein
(ATF-1, ATF2, HFB1-GDNF)
Prod. No.: G715
Source NSO Cells
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C

Description
Background:

Glial cell derived neurotrophic factor, also known as GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF potently promotes the survival of many types of neurons, most prominently, dopaminergic and motorneurons. GDNF regulates kidney development and spermatogenesis, and it affects alcohol consumption.2

Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human GDNF is Mr 11.6 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GDNF was determined by its ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia neurons (Davies, A.M., 1989, Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor, Rush, R.A., ed., John Willey and Sons, Ltd., p.95). The expected ED50 for this effect is typically 1 - 3 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

rg qrgknrgcvl taihlnvtdl glgyetkeel ifrycsgscd aaettydkil knlsrnrrlv sdkvgqaccr piafdddlsf lddnlvyhil rkhsakrcgc i

 

References
1. Collins F. et al. (1993)Science 260: 1130
2. Ron D et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105: 8114
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