产品中心

产品搜索 抗体高级搜索

首页 > 产品列表 > 细胞因子/重组蛋白/抗原 > 重组与天然蛋白质

Recombinant Human GDF-3

产品编号:G584-1

产品描述:Recombinant Human GDF-3

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:订购

说明书:

Recombinant Human Growth/Differentiation Factor-3 (GDF-3)
(Vgr-2, UNQ222/PRO248)
Prod. No.: G584
Source E. Coli
Pkg. Size: 1 mg, 20 µg 

Description
Background:

Growth differentiation factor-3 (GDF3) also known as Vg-related gene 2 (Vgr-2), is a protein belonging to the transforming growth factor beta (TGF-β) superfamily. It has high similarity to other TGF-β superfamily members called Vg1, BMP2, BMP6 and GDF1, but lacks one of the conserved cysteine residues common to all other TGF-β superfamily members.1 Expression of GDF3 occurs in ossifying bone during embryonic development and in the brain, thymus, spleen, bone marrow and adipose tissue of adults.2,3
GDF3 is a bi-functional protein that inhibits some TGF-β superfamily members (e.g. BMPs) while activating others, thus regulating the balance between different modes of TGF-beta signaling.4 GDF3 regulates both of the two major characteristics of embryonic stem cells: the ability to maintain the undifferentiated state and the ability to differentiate into the full spectrum of cell types.5

Source
E. Coli
State of Matter
 
Purity
>98% by SDS Page and HPLC
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg
Biological Activity
The biological activity of Human Growth/Differentiation Factor-3 is determined by its ability to inhibit induced alkaline phosphatase production by ATC\DC-5 chondrogenic cells. The expected ED50 for this effect is 100-150 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence

AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

References
1. van den Eijnden-van Raaij A et al. (1998) Oncogene 16: 95
2. Brown C et al. (2006) Development 133: 319
3. Yu L et al. (2005) Neurosci Lett 389: 83
4. Brivanlou A et al. (2006) Cell Cycle 5: 1069
5. Brivanlou AH. et al. (2006) Developement 133: 209
Related Products
Anti-Human GDF-3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G593)
Anti-Human GDF-3 (Antigen Aff. Pur.) (Leinco Prod. No.: G592)
Recombinant Human GDF-3 (Leinco Prod. No.: G710)