产品编号:F116-25
产品描述:Recombinant Human Fractalkine(CX3CL1)
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Human Fractalkine (FKN)
Purified No Carrier Protein
(CX3CL1, NTN, ABCD-3, C3Xkine, CXC3, CXC3C, NTT, SCYD1, Neurotactin, FKN)
Prod. No.: F116
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium1 that mediates attachment and firm adhesion of T cells, monocytes and NK cells.2 FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.3 FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).4
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human Fractalkine is Mr 35 kDa. However, due to glycosylation, the actual molecular weight as observed by migration on SDS Page is Mr 90 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Fractalkine was determined by its ability to chemoattract freshly isolated peripheral blood lymphocytes or mouse Baf/3 cells transfected with hCX3CR-1. The expected ED50 for this effect is typically 50 - 150 ng/ml, or 2.5 - 10 ng/ml, respectively.
Each investigator should determine their own optimal working dilution for specific applications.
References
1. Patel DD et al. (1998) J Exp Med. 188: 1413
2. Hwang ST et al. (1999) Eur J Immunol. 29: 2551
3. Muller GA et al. (2008) Z Rheumatol. 67: 424
4. Mori M et al. (2005) Int J Oncol. 26: 41
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