产品编号:F114-1
产品描述:Recombinant Human Flt-3 Ligand
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Flt-3 Ligand (FLT3LG)
Purified No Carrier Protein
(Fms-Related Tyrosine Kinase 3 Ligand, Flk-2 Ligand, FL, FLT3LG, STK-1 Ligand)
Prod. No.: F114
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.3
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human FLT3LG is Mr 17.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 17 - 30 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human flt3-Ligand was determined by its ability to stimulate the proliferation of a mouse Flt3 transfected BaF/3 cell line. The expected ED50 for this effect is typically 0.2 - 1 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
tqdc sfqhspissd favkirelsd yllqdypvtv asnlqdeelc galwrlvlaq rwmerlktva gskmqgller vnteihfvtk cafqpppscl rfvqtnisrl lqetseqlva lkpwitrqnf srclelqcqp dsstlpppws prpleatapt apqpp
References
1. Mattson J et al. (1994) Nature 368: 643
2. Cleveland LS et al. (1995) Oncogene 10: 149
3. C.R. Maliszewski et al. (1998) J. Exp. Med. 188: 2075
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