产品编号:F1027-1
产品描述:Recombinant Rat FGF-basic
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Rat Fibroblast Growth Factor-Basic (FGF-Basic)
Purified No Carrier Protein
(B-FGF, FGF-2, FGFB, Prostatropin, NUDT6)
Prod. No.: F1027
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that exert pleiotropic effects in a range of cell types including skeletal myocytes.1 Fibroblast growth factor basic (FGF basic) significantly promote angiogenesis; a central event for the growth and metastasis of solid tumors.2 It is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. FGF-basic plays a central role during prenatal development and postnatal growth and regeneration of tissues, by promoting cellular proliferation and differentiation. FGF-basic is one of the angiogenesis factors and may promote wound healing.3
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Rat FGF-Basic is Mr 16.2 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, sodium sulphate (Na2SO4), EDTA, Dithiothreitol (DTT).
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein such as 0.1% BSA or HSA, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Rat FGF-Basic was determined by thymidine uptake by 3T3 cells expressing FGF receptors. The expected ED50 is 0.1 - 10 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
alpedgggaf ppghfkdpkr lycknggffl rihpdgrvdg vreksdphvk lqlqaeergv vsikgvcanr ylamkedgrl laskcvteec ffferlesnn yntyrsrkys swyvalkrtg qyklgsktgp gqkailflpm saks
References
1. Swain JL et al. (1991) Developement 111: 741
2. Grevers G et al. (1997) Laryngorhinootologie 76: 421
3. (2007) ORG Genetics
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