产品编号:F110-25
产品描述:Recombinant Human FGF-basic
反应种属:
实验方法:
标记:
规格:25ug
供应商:Leinco
价格(RMB):3750.00
订购:
说明书:
Recombinant Human Fibroblast Growth Factor-Basic (FGF-Basic)
Purified No Carrier Protein
(B-FGF, FGF-2, FGF-β, FGFB, Prostatropin, NUDT6)
Prod. No.: F110
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Basic fibroblast growth factor (bFGF), also known as FGF-2 and FGF-β, is a non-glycosylated heparin binding growth factor and member of the FGF family of mitogenic proteins. Members of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation (1). bFGF is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. It is secreted by mechanisms other than the classical protein secretion pathway due to the lack of a signal peptide. Acidic FGF (aFGF) and bFGF bind to the same high affinity receptors (2). Binding of bFGF to heparin or cell surface heparan sulfate proteoglycans is a prerequisite for ligation of bFGF to these receptors. aFGF and BFGF have a similar range of biological activities which are implicated in several important physiological and pathological processes, such as embryonic development and differentiation, morphogenesis, angiogenesis, and wound healing (3-4).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human FGF-Basic is Mr 17.4 kDa.
State of Matter
Solution
Formulation
This recombinant protein solution was 0.2 µm filtered and formulated in Tris and NaCl.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This protein is stable for > twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-basic was monitored in a mitogenic assay by measuring the FGF basic dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Rizzino, A. et al., 1988. Cancer Research 48:4266 - 4271). The expected ED50 for this effect is typically 0.1 - 0.25 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
gtmaagsit tlpalpedgg sgafppghfk dpkrlyckng gfflrihpdg rvdgvreksd phiklqlqae ergvvsikgv canrylamke dgrllaskcv tdecffferl esnnyntyrs rkytswyval krtgqyklgs ktgpgqkail flpmsaks
References
1. Swain, J. L. et al. (1991) Development 111:741
2. Dionne, C. A. et al. (1990) EMBO J. 9:2685
3. Fiddes, J. C. et al. (1991) Ann. N. Y. Acad. Sci. 638:316
4. Yamanaka, Y. et al. (1993) Cancer Res. 53:5289
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