产品编号:F125-1
产品描述:Recombinant Human FGF-16
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Fibroblast Growth Factor-16 (FGF-16)
Purified No Carrier Protein
(FGFG)
Prod. No.: F125
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Fibroblast growth factor 16 (FGF-16) is a member of the fibroblast growth factor (FGF) family, which are all heparin-binding growth factors. FGF-16 is most similar to FGF-9 sharing 73% amino acid identity. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-16 is released from neonatal cardiac myocytes and alters growth-related signaling, possibly interfering with cardiac myocyte proliferative potential (2). It lacks a typical signal peptide but is efficiently generated by mechanisms other than the classical protein secretion pathway. FGF-16 signals through the receptors FGFR 2c and 3c (3). It induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4).
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human FGF-16 is Mr 23.7 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, EDTA, Dithiothreitol (DTT), and sodium sulphate (Na2SO4).
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-16 was determined by its ability stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Rizzino, A. et al., 1988, Cancer Research 48:4266 - 4271; Thomas, K., 1987, in Methods in Enzymology 147:120 - 135). The expected ED50 for this effect is typically 7.5 - 30 ng/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
aevggvfas ldwdlhgfss slgnvplads pgflnerlgq iegklqrgsp tdfahlkgil rrrqlycrtg fhleifpngt vhgtrhdhsr fgilefisla vglisirgvd sglylgmner gelygskklt recvfreqfe enwyntyast lykhsdserq yyvalnkdgs pregyrtkrh qkfthflprp vdpsklpsms rdlfhyr
References
1. Cattini, P. A. et al. (2003) Mol. Cell. Biochem. 242:65
2. Lu, S. Y. et al. (2008) Amer. J. Physiol. 294:1242
3. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694
4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119
Related Products
Anti-Human FGF-16-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F162)
Anti-Human FGF-16 (Antigen Aff. Pur.) (Leinco Prod. No.: F161)
