产品编号:F124-1
产品描述:Recombinant Human FGF-10
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Fibroblast Growth Factor-10 (FGF-10)
Purified No Carrier Protein
(FGFA, Keratinocyte Growth Factor-2, Repifermin)
Prod. No.: F124
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C
Description
Background:
Fibroblast growth factor 10, also known as FGF10, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is a primary factor in the process of wound healing similarly to other growth factors such as TGF alpha and FGF7.1
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human FGF-10 is Mr 19.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 19 kDa.
State of Matter
Lyophilized
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-10 was determined by its ability to stimulate the proliferation of a monkey epithelial cell line, 4MBr-5 (Rubin, J.S. et al., 1989, Proc. Natl. Acad. Sci. USA 86:802). The expected ED50 for this effect is typically 100 - 300 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
cqal gqdmvspeat nsssssfssp ssagrhvrsy nhlqgdvrwr klfsftkyfl kiekngkvsg tkkencpysi leitsveigv vavkainsny ylamnkkgkl ygskefnndc klkerieeng yntyasfnwq hngrqmyval ngkgaprrgq ktrrkntsah flpmvvhs
References
1. Nakatsuka M. et al. (1997) Gene197: 399
Related Products
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Anti-Human FGF-10 (Antigen Aff. Pur.) (Leinco Prod. No.: F151)
Recombinant Human FGF-10 (Leinco Prod. No.: K131)
