产品编号:F123-25
产品描述:Recombinant Human FGF-9
反应种属:
实验方法:
标记:
规格:25µg
供应商:Leinco
价格(RMB):3750.00
订购:
说明书:
Recombinant Human Fibroblast Growth Factor-9 （FGF-9）
Purified No Carrier Protein
（Growth Factor-9, GAF （Glia-Activating Factor）, HBGF-9, HBFG-9, MGC119914, MGC119915）
Prod. No.: F123
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Fibroblast growth factor 9 （glia-activating factor）, also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.1 It is a member of the fibroblast growth factor （FGF） family that possess broad mitogenic ａnd cell survival activities, ａnd are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth ａnd invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons ａnd may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.2 It is highly related to FGF-16.
FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 ａnd the FGFR3b ａnd FGFR3c splice isoforms of FGFR3.3 FGF-9 is a high affinity, heparin dependent ligand for FGFR3 ａnd FGFR2 but not for FGFR1 ａnd FGFR4.4
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human FGF-9 is Mr 23 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 22-29 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-9 was determined by its ability to stimulate proliferation of a mouse fibroblast cell line, Balb/3T3 （Rubin, J.S. et al., 1991, Proc. Natl. Acad. Sci. USA 88:415）. The expected ED50 for this effect is typically 1 - 5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
maplgevgny fgvqdavpfg nvpvlpvdsp vllsdhlgqs eagglprgpa vtdldhlkgi lrrrqlycrt gfhleifpng tiqgtrkdhs rfgilefisi avglvsirgv dsglylgmne kgelygsekl tqecvfreqf eenwyntyss nlykhvdtgr ryyvalnkdg tpregtrtkr hqkfthflpr pvdpdkvpel ykdilsqs
Country of Origin
USA
References
1. Imamura T. et al. （1999）J Biol Chem 274: 29352
2. Miyamoto M et al. （1993）Molecular Cellular Biology 13: 4251
3. Santos-Ocampo S et al. （1996）Journal of Biological Chemistry 271: 1726
4. Hecht D et al. （1995）Growth Factors 12: 223
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