产品编号:F111-2
产品描述:Recombinant Mouse FGF-9
反应种属:
实验方法:
标记:
规格:2µg
供应商:Leinco
价格(RMB):2175.00
订购:
说明书:
Recombinant Mouse Fibroblast Growth Factor-9 （FGF-9）
（Growth Factor-9, GAF （Glia-Activating Factor）, HBGF-9, HBFG-9, MGC119914, MGC119915）
Prod. No.: F111
Source E. coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Description
Background:
Fibroblast growth factor 9 （glia-activating factor）, also known as FGF9 is a glycosylated neurotrophic polypeptide highly expressed in brain.1 It is a member of the fibroblast growth factor （FGF） family that possess broad mitogenic ａnd cell survival activities, ａnd are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth ａnd invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons ａnd may be important for glial cell development. FGF-9 has sequence similarity of approximately 30% to other members of the family of fibroblast growth factors.2 It is highly related to FGF-16.
FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 ａnd the FGFR3b ａnd FGFR3c splice isoforms of FGFR3.3 FGF-9 is a high affinity, heparin dependent ligand for FGFR3 ａnd FGFR2 but not for FGFR1 ａnd FGFR4.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Mouse FGF-9 is Mr 23 kda kDa.
State of Matter
Lyophilized
Purity
>95% by SDS Page ａnd HPLC
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes ａnd store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg
Biological Activity
The biological activity of Mouse Fibroblast Growth Factor-9 is determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 for this effect is <0.5 ng/ml, corresponding to a specific activity of >2 x 106 units/mg.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS
Country of Origin
USA
References
1. Imamura T. et al. （1999）J Biol Chem 274: 29352
2. Miyamoto M et al. （1993）Molecular Cellular Biology 13: 4251
3. Santos-Ocampo S et al. （1996）Journal of Biological Chemistry 271: 1726
4. Hecht D et al. （1995）Growth Factors 12: 223
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Recombinant Human FGF-9 （Leinco Prod. No.: F123）
Anti-Human FGF-9 （Leinco Prod. No.: F1070）
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