产品编号:F137-50

产品描述:Recombinant Human FGF-5

反应种属:

实验方法:

标记:

规格:50µg

供应商:Leinco

价格(RMB):3000.00

订购:

说明书:

Recombinant Human Fibroblast Growth Factor-5 （FGF-5）
Purified No Carrier Protein
（HBGF-5, Smag-82）
Prod. No.: F137
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Fibroblast growth factor 5, also known as FGF5 is a secreted signaling protein1 that is a member of the fibroblast growth factor gene family.2 FGF5 is widely expressed in embryonic but scarcely in adult tissues.3 FGF5 contributes to the malignant progression of human astrocytic brain tumours by both autocrine ａnd paracrine effects.3 FGF-5 inhibitors are promising substances against hair loss and/or for promoting hair growth.4 FGF5 may also have a functional role in the pathophysiology of age-related macular degeneration （ARMD）.5

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human FGF-5 is Mr 27 kDa.
State of Matter
Solution
Formulation
The protein is provided in a 0.2 μm filtered solution of 20 mM MOPS, 50 mM Na2SO4, 0.2 mM EDTA, 0.5 mM DTT, pH 7.0 at a concentration of 0.24 mg/ml.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This protein is stable for >12 months when stored at -20°C to -70°C in a manual defrost freezer. After thawing ａnd addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for up to three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-5 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts （Thomas, K. 1987, Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266）. The expected ED50 for this effect is typically 2 - 10 ng/ml in the presence of 1 μg/ml of heparin.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
ekrlapkgqp gpaatdrnpr gsssrqssss amssssasss paaslgsqgs gleqssfqws psgrrtgsly crvgigfhlq iypdgkvngs heanmlsvle ifavsqgivg irgvfsnkfl amskkgklha sakftddckf rerfqensyn tyasaihrte ktgrewyval nkrgkakrgc sprvkpqhis thflprfkqs eqpelsftvt vpekkkppsp ikpkiplsap rkntnsvkyr lkfrfg

Country of Origin
USA
References
1. Martin GR et al. （1997） Cell 78: 1017
2. Thoenen H et al. （1994） Eur J Neurosci. 6: 244
3. Berger W et al. （2008） Oncogene 27: 4180
4. Suzuki S et al. （2003） J Cell Physiol. 197: 272
5. Hjelmeland LM et al. （1997） Curr Eye Res. 16: 396
Related Products
Anti-Human FGF-5 （Leinco Prod. No.: F155）
Anti-human FGF-5-Biotin （Antigen Aff. Pur.） （Leinco Prod. No.: F1091）