产品编号:F128-25

产品描述:Recombinant Human FGF-4

反应种属:

实验方法:

标记:

规格:25µg

供应商:Leinco

价格(RMB):5325.00

订购:

说明书:

Recombinant Human Fibroblast Growth Factor-4 （FGF-4）
Purified No Carrier Protein
（HST-1, Transforming Protein KS3, HBGF-4, HST, HSTF1, K-FGF, KFGF）
Prod. No.: F128
Source E. coli
Pkg. Size: 1 mg, 5 µg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Fibroblast growth factor 4 （heparin secretory transforming protein 1, Kaposi sarcoma oncogene）, also known as FGF4 is a member of the fibroblast growth factor （FGF） family. FGF family members possess broad mitogenic ａnd cell survival activities ａnd are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth ａnd invasion. FGF-4 has an ability to promote neural stem cell proliferation ａnd neuronal differentiation in the postnatal brain.1

Source
E. coli
Molecular Weight
This preparation of Recombinant Human FGF-4 is subject to proteolytic cleavage, which results in a mixture of 4 peptides ａnd reveals bands on an SDS PAGE gel at Mr 14 kDa & 16 kDa.
State of Matter
Lyophilized
Purity
>95% by SDS-PAGE ａnd HPLC
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FGF-4 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts （Thomas, K., 1987 Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266）. The expected ED50 for this effect is typically 0.05 - 0.15 ng/mL.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
The DNA sequence encoding the putative mature 176 amino acid residue FGF-4 （excluding the 30 amino acid residue deduced signal peptide） was cloned into a suitable expression vector ａnd expressed in E. coli （Delli Bovi, P. et al., 1987, Cell 50:729 - 737; Miyagawa, K. et al., 1988, Oncogene 3:383 - 389）.

Country of Origin
USA
References
1. Ochiya T. et al. （2006） FASEB J. 20: 1848
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