产品编号:E212-1
产品描述:Recombinant Mouse Ephrin-A1
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
说明书:
Recombinant Mouse Ephrin-A1
Purified No Carrier Protein
(B61, LERK-1, EFL-1)
Prod. No.: E212
Source NSO Cells
Pkg. Size: 1 mg, 200 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse Ephrin-A1 is Mr 46.8 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 50-55 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse Ephrin-A1 was determined its ability to bind in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 μg/ml (100 μl/well) can bind rmEphrinA1/Fc Chimera with a linear range of 0.16 - 10 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
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