产品编号:E118-25

产品描述:Recombinant Human Eotaxin-3

反应种属:

实验方法:

标记:

规格:25µg

供应商:Leinco

价格(RMB):5100.00

订购:

说明书:

Recombinant Human Eotaxin-3
Purified No Carrier Protein
（SCYA26, MIP-4 Alpha, Eotaxin-3, IMAC, MIP-4a, TSC-1, CCL26）
Prod. No.: E118
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Eotaxin-3/CCL-26 belongs to a family of CC chemokines, which are potent chemoattractants for eosinophils that signal through the CCR3 receptor. 1 It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells ａnd monocytes. It has been reported that eotaxin-3 is an antagonist for CCR2.2 Eotaxin-3 has similar activity to Eotaxin ａnd Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer ａnd activator of transcription 6, thus suggesting that the intestinal epithelium actively participates in the recruitment of eosinophils at the site of inflammation.1

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human Eotaxin-3 is Mr 8.4 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile （CH3CN） ａnd 0.1% trifluoroacetic acid （TFA）.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Eotaxin-3 was determined by its ability to induce chemotaxis of mouse BaF/3 cells stably expressing mCCR3. The expected ED50 for this effect is typically 0.2 - 0.8 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
trgsdis ktccfqyshk plpwtwvrsy eftsnscsqr avifttkrgk kvcthprkkw vqkyisllkt pkql

Country of Origin
USA
References
1. Cuber JC.et al. （2005） Int J Biochem Cell biol. 37: 2559
2. Gerber B. et al. （2004） J Biol Chem. 279: 23357
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