产品编号:C1355-50
产品描述:Recombinant Mouse E-Cadherin
反应种属:
实验方法:
标记:
规格:50µg
供应商:Leinco
价格(RMB):4725.00
订购:
说明书:
Recombinant Mouse E-Cadherin （E-CAD）
Purified No Carrier Protein
（Cell-CAM120/80, Uvomorulin, Arc-1, L-CAM, CDH1, CD324, CDHE, UVO）
Prod. No.: C1355
Source NSO Cells
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Cadherin 1, type 1, E-cadherin （epithelial）, also known as CDH1 ａnd CD32, is a tumor suppressor gene. 1, 2 The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region ａnd a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, ａnd renal-cell carcinoma. 3
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse ECAD is Mr 88.2 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl ａnd 2 mM CaCl2, pH 6.5.
Purity
>90% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 104 cells/well are added to rmECAD/Fc Chimera coated plates （1.5 μg/ml with 100 μl/well）, approximately 50-70% will adhere after 90 minutes at room temperature.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
References
1. Christofori G et al.（1998） Am. J. Hum. Genet. 63: 1588
2. Gumbiner BM et al.（2003） J. Cell Biol.161: 1191
3. Isaacs WB et al.（1995） World J Urol.13:364
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