产品编号:C1319-1
产品描述:Recombinant Human CTLA-4
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen （CTLA-4）
Purified No Carrier Protein
（CD152, CELIAC3, GSE, IDDM12）
Prod. No.: C1319
Source Sf 21 Insect Cells
Pkg. Size: 1 mg, 200 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
CTLA4 （Cytotoxic T-Lymphocyte Antigen 4） also known as CD152, is a protein which is expressed on the surface of Helper T cells ａnd plays an important regulatory role in the immune system.1 CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells.2,3 CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis ａnd may also be useful during organ transplantation ａnd cancer treatment.
Source
Sf 21 Insect Cells
Molecular Weight
The predicted molecular weight of Recombinant Human CTLA-4 is Mr 40 kDa （monomer）.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CTLA-4 was determined by its ability to inhibit IL-2 production by Jurkat cells stimulated with Raji cells or 1 μg/ml rhB7-1/Fc in the presence of PHA （Linsley, P.S. et al., 1991, J. Exp. Med. 174:561）. The expected ED50 is typically 2 - 4 μg/ml with Raji cells or 0.1 - 0.4 μg/ml with rhB7-H1/Fc.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
amhv aqpavvlass rgiasfvcey aspgkatevr vtvlrqadsq vtevcaatym mgneltfldd sictgtssgn qvnltiqglr amdtglyick velmypppyy lgigngtqiy vidpepcpds dfiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgkh hhhhh
Country of Origin
USA
References
1. Layrisse Z et al. （2009） Hum Immunol.
2. Mak TW et al. （1995） Science 270: 985
3. Delneste Y et al. （1999） Eur. J. Immunol 29: 3596
4. Vijayakrishnan L et al. （2004） Immunity 20: 563
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