产品编号:C727-10
产品描述:Recombinant Human CTGF
反应种属:
实验方法:
标记:
规格:10µg
供应商:Leinco
价格(RMB):4275.00
订购:
说明书:
Recombinant Human Connective Tissue Growth Factor （CTGF）
（CCN2, Hypertrophic Chondrocyte-Specific Protein 24 [HCS24], IGFBP8, MGC102839, NOV2）
Prod. No.: C727
Source E. Coli
Pkg. Size: 1 mg, 2 µg, 10 µg, 100 µg
Storage: -20°C Detailed storage instructions below.
Description
Background:
CTGF （connective tissue growth factor） is a cysteine-rich, matrix-associated, heparin-binding protein ａnd a member of the CCN family of secreted cysteine rich regulatory proteins ａnd is the major mitogenic ａnd chemoattractant protein produced by umbilical vein ａnd vascular endothelial cells. CTGF stimulates the proliferation ａnd differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, ａnd epithelial cells, ａnd binds to IGF, TGF beta1, ａnd BMP-4. Cell migration ａnd adhesion are signaled through binding to specific cell surface integrins ａnd to heparin sulfate proteoglycans CTGF, a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, ａnd mitogenic CTGF activity.
Source
E. Coli
State of Matter
Lyophilized
Purity
>98% by SDS-PAGE ａnd HPLC
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes ａnd store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg
Biological Activity
The biological activity of Human Connective Tissue Growth Factor is determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Country of Origin
USA
Related Products
Human CTGF ELISA Development Kit （Leinco Prod. No.: C1587）
