产品编号:C387-1
产品描述:Recombinant Human CNTF
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Ciliary Neurotrophic Factor （CNTF）
Purified No Carrier Protein
（HCNTF）
Prod. No.: C387
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Ciliary neurotrophic factor （CNTF）, also known as HCNTF, is a cytokine that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic ａnd motoneurons （1）. It is structurally related to IL-6, IL-11, LIF ａnd OSM. CNTF is expressed in glial cells within the central ａnd peripheral nervous systems. It lacks the classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release by some mechanism induced by injury. The receptors for CNTF are CNTF Rα subunit ａnd gp130, which are expressed exclusively in the nervous system ａnd skeletal muscle. In addition to its neuronal actions, CNTF also acts on non-neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells ａnd bone marrow stromal cells. Because CNTF is predominantly expressed in the nervous system, its modulation may have implications for Parkinson＇s disease, ALS ａnd cell-replacement therapies for other disorders （2-3）. CNTF mimics the biological actions of leptin while overcoming "leptin resistance" ａnd appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver ａnd skeletal muscle （4）. Therefore, it may be a therapeutic target for obesity-related metabolic disease. CNTF is highly conserved across species ａnd exhibits cross-species bioactivity.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CNTF is Mr 22.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 23 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） ａnd cysteine pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes ａnd store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CNTF was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 （Kitamura, T. et al., 1989, J. Cell Physiol. 140:323）. The expected ED50 is typically 50 - 150 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
References
1. Thoenen, H. et al. （1994） J. Neurobiol. 25:1436
2. Hagg, T. et al. （2008） J. Neurosci. 28:2231
3. Masu, Y. et al. （1993） Nature 365:27
4. Febbraio, M. A. et al. （2008） J. Mol. Med. 86:3
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