Recombinant Rat Ciliary Neurotrophic Factor (CNTF)
Purified No Carrier Protein
(HCNTF)
| Prod. No.: |
C329 |
| Source |
E. coli |
| Pkg. Size: |
1 mg, 10 µg, 50 µg |
| Storage: |
-20°C to -70°C Detailed storage instructions below. |
Description
Background:
Ciliary neurotrophic factor (CNTF) is a cytokine1 that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic, and motoneurons.2 With these abilities, CNTF has implications for Parkinson's disease and cell-replacement therapies for other disorders.3 CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle. The advantages and disadvantages of CNTF has shown promise as a therapeutic strategy to alleviate obesity-associated diseases.4
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Rat CNTF is Mr 22.8 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
afaeqtplt lhrrdlcsrs iwlarkirsd ltalmesyvk hqglnkninl dsvdgvpvas tdrwsemtea erlqenlqay rtfqgmltkl ledqrvhftp tegdfhqaih tlmlqvsafa yqleelmvll eqkipenead gmpatvgdgg lfekklwglk vlqelsqwtv rsihdlrvis shqmgisale shygakdkqm
Country of Origin
USA
References
1. Boschero AC et al. (2009) Cytokine
2. Thoenen H et al. (1994) J Neurobiol. 25: 1436
3. Hagg T et al. (2008) J Neurosci. 28: 2231
4. Febbraio MA et al. (2008) J Mol Med. 86: 353
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Anti-Rat CNTF-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C397)
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