产品编号:N138-1

产品描述:Recombinant Human CLC

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:

说明书:

Recombinant Human Cardiotrophin-Like Cytokine （CLC）
Purified No Carrier Protein
（NR6, B Cell Stimulating Factor-3 （BSF3）, Novel Neurotrophin-1 （NNT1）, CISS2, CLCF1）
Prod. No.: N138
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Cardiotrophin-like cytokine （CLC） also known as novel neurotrophin-1/B cell stimulating factor-3 （NNT-1/BSF-3） is a member of the interleukin （IL）-6 family of cytokines.1 CLC is expressed highly in spleen ａnd peripheral leukocytes2 ａnd is a target-derived factor required for the survival of specific pools of motoneurons.3 CLC shows neuroprotective effects on retinal ganglion cells （RGCs） in vivo ａnd might be a treatment option for chronic neurodegenerative eye diseases such as glaucoma.4

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CLC is Mr 22.4 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 40% acetonitrile （CH3CN） ａnd 0.1% trifluoroacetic acid （TFA）.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<0.1 ng per 1 µg as determined by the LAL method
Biological Activity
The biological activity of Human CLC was determined in a cell proliferation assay using a CNTFRα transfected cell line, TF-1.CN5a.1. The expected ED50 for this effect is typically 0.05 - 0.25 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
lnrgdpgpgp siqktydltr ylehqlrsla gtylnylgpp fnepdfnppr lgaetlprat vdlevwrsln dklrltqnye ayshllcylr glnrqaatae lrrslahfct slqgllgsia gvmaalgypl pqplpgtept wtpgpahsdf lqkmddfwll kelqtwlwrs akdfnrlkkk mqppaaavtl hlgahgf

Country of Origin
USA
References
1. Taga T et al. （2002） Cytokine 18: 1
2. Shi Y et al. （1999） Biochemical Biophysical Res Communications 262: 132
3. Oppenheim RW et al. （2003） J Neurosci. 23: 8854
4. Rong Wen et al. （2005） Graefe＇s Archive for Clinical ａnd Experimental Ophthalmol. 243: 1036
Related Products
Anti-Human CLC （Antigen Aff. Pur.） （Leinco Prod. No.: N147）
Anti-Human CLC （Leinco Prod. No.: C1462）
Anti-Human CLC-Biotin （Antigen Aff. Pur.） （Leinco Prod. No.: N148）