产品编号:C1339-1
产品描述:Recombinant Human CD27
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Cluster of Differentiation 27 （CD27）
Purified No Carrier Protein
（MGC20393, S152, T14, TNFRSF7, Tp55）
Prod. No.: C1339
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
CD27 is a tumor necrosis factor receptor. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation ａnd long-term maintenance of T cell immunity. It exists as both a disulfide-linked dimer on the cell surface ａnd as a soluble protein found in serum. It binds to ligand CD70 which is expressed on thymic stromal cells ａnd a small subset of activated T cells ａnd plays a key role in regulating B-cell activation ａnd immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB ａnd MAPK8/JNK. Adaptor proteins TRAF2 ａnd TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein （SIVA）, a proapoptotic protein, can bind to this receptor ａnd is thought to play an important role in the apoptosis induced by this receptor.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human CD27 is Mr 46.5 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 60-70 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>90% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CD27 was determined by its ability to inhibit the proliferation of mouse T cells induced by recombinant mouse CD27 ligand （10 μg/ml, 100 μl/well） ａnd a sub-optimal concentration of anti-CD3. The expected ED50 is typically 0.75 - 3 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
