产品编号:C841-25

产品描述:Recombinant Human CCL27

反应种属:

实验方法:

标记:

规格:25µg

供应商:Leinco

价格(RMB):5325.00

订购:

说明书:

Recombinant Human Chemokine （C-C Motif） Ligand 27 （CCL27）
Purified No Carrier Protein
（ALP, ILC, Eskine, Cutaneous T-Cell Attracting Chemokine, CTACK, SCYA27, Skinkine, PESKY）
Prod. No.: C841
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Chemokine （C-C motif） ligand 27 （CCL27） is a small cytokine belonging to the CC chemokine family. It is associated with homing of memory T lymphocytes to the skin, ａnd plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta ａnd skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10.

CCL27 is one of the CC chemokines produced by epidermal keratinocytes ａnd is suggested to be involved in the pathogenesis of inflammatory skin diseases. CCL27 modifies inflammation by attracting CCR10-positive ａnd CCR4-positive cells into the lesional skin, ａnd may participate in the pathogenesis of Th2-shifted skin diseases such as atopic dermatitis.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human CTACK is Mr 10.1 kDa. Based on mass spectrometry, a carboxyl-terminal truncated form of the protein having a molecular weight of ~9.8 kDa is also present in the preparation.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile （CH3CN） ａnd 0.1% trifluoroacetic acid （TFA）.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CTACK was determined by its ability to induce chemotaxis of mouse BaF/3 cells transfected with mCCR10. The expected ED50 for this effect is typically 0.1 - 0.4 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
fllppstacc tqlyrkplsd kllrkviqve lqeadgdchl qafvlhlaqr sicihpqnps lsqwfehqer klhgtlpkln fgmlrkmg

Country of Origin
USA
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