产品编号:B534-1
产品描述:Recombinant Human BCAM
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Basal-Cell Adhesion Molecule （BCAM）
Purified No Carrier Protein
（AU, CD239, LU, MSK19）
Prod. No.: B534
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Basal cell adhesion molecule （BCAM）, also known as CD239, ａnd Lutheran blood group glycoprotein （LU） are two alternatively spliced variants of a single pre-mRNA. They are type I membrane proteins of the immunoglobulin superfamily （IgSF） sharing similar structure characteristics except for the length of their cytoplasmic tails （1）. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium ａnd the endothelium of blood vessel walls （2）. BCAM ａnd LU share a significant sequence similarity with the CD146 （MUC18） ａnd CD166, ａnd themselves are adhesion molecules that bind laminin with high affinity （3）. It has been shown that BCAM expression is upregulated following malignant transformation in some cell types in vivo ａnd in vitro, ａnd BCAM is identified as a marker of epithelial ovarian cancers. In addition, BCAM interacts with integrin in sickle red cells, ａnd thus may potentially play a role in vaso-occlusive episodes （4）. It has been demonstrated that expression of BCAM is inversely associated with the susceptibility of cells to cell death by apoptosis （5）. Overexpression of the protein or inhibition of BCAM by suitable antibodies does not influence apoptotic processes.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human BCAM is Mr 83.7 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 110-120 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
evrlsvppl vevmrgksvi ldctptgthd hymlewfltd rsgarprlas aemqgselqv tmhdtrgrsp pyqldsqgrl vlaeaqvgde rdyvcvvrag aagtaeatar lnvfakpeat evspnkgtls vmedsaqeia tcnsrngnpa pkitwyrngq rlevpvemnp egymtsrtvr easgllslts tlylrlrkdd rdasfhcaah yslpegrhgr ldsptfhltl hyptehvqfw vgspstpagw vregdtvqll crgdgspspe ytlfrlqdeq eevlnvnleg nltlegvtrg qsgtygcrve dydaaddvql sktlelrvay ldplelsegk vlslplnssa vvncsvhglp tpalrwtkds tplgdgpmls lssitfdsng tyvceaslpt vpvlsrtqnf tllvqgspel ktaeiepkad gswregdevt licsarghpd pklswsqlgg spaepipgrq gwvsssltlk vtsalsrdgi sceasnphgn krhvfhfgav spqtsqadie grmdpkscdk thtcppcpap ellggpsvfl fppkpkdtlm isrtpevtcv vvdvshedpe vkfnwyvdgv evhnaktkpr eeqynstyrv vsvltvlhqd wlngkeykck vsnkalpapi ektiskakgq prepqvytlp psrdeltknq vsltclvkgf ypsdiavewe sngqpennyk ttppvldsdg sfflyskltv dksrwqqgnv fscsvmheal hnhytqksls lspgkhhhhh h tyvceaslpt vpvlsrtqnf tllvqgspel ktaeiepkad gswregdevt licsarghpd pklswsqlgg spaepipgrq gwvsssltlk vtsalsrdgi sceasnphgn krhvfhfgav spqtsqa
Country of Origin
USA
References
1. Campbell, I. G. et al. （1994） Cancer Res. 54:5761
2. Parsons, S. F. et al. （1995） Proc. Natl. Acad. Sci. （USA） 92:5496
3. Nemer, W. E. et al. （2001） J. Biol. Chem. 276:23757
4. Nemer, W. E. et al. （2007） Blood 109:3544
5. Drewniok, C. et al. （2004） Skin Pharmacol. Physiol. 17:304
Related Products
Anti-Human BCAM （Antigen Aff. Pur.） （Leinco Prod. No.: B559）
Anti-Human BCAM-Biotin （Antigen Aff. Pur.） （Leinco Prod. No.: B604）
