产品编号:A409-1

产品描述:Recombinant Human Bcl-2-related Protein A1

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:

说明书:

Recombinant Human Bcl-2-related Protein A1 （BCL2A1）
Purified No Carrier Protein
（Bfl-1, GRS）
Prod. No.: A409
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Bcl-2 related protein A1 （Bcl2A1） is a member of the Bcl-2 family of proteins that regulate outer mitochondrial membrane permeability （1）. The proteins of this family act as anti- ａnd pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis ａnd tumorigenesis. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bcl2A1, which is overexpressed in stomach ａnd other cancers. Bcl2A1 can interact with Bax ａnd suppress apoptosis by inhibiting the release of cytochrome c ａnd caspase-3 activation （2）. It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance ａnd inhibition of apoptosis in cancer cells. At least two isoforms of Bcl2A1 are known to exist.

Source
E. coli
Molecular Weight
The molecular weight of Recombinant human BCL2A1 migrates as a monomeric 18 kDa polypeptide on SDS-reducing ａnd non-reducing PAGE. Amino acid sequencing shows that the amino terminal methionine was removed during expression in E. coli.
State of Matter
Solution
Formulation
This recombinant protein was 0.2 µm filtered ａnd is supplied as a solution of 20 mM HEPES （pH 8.5）, 0.1 M KCl ａnd 20% glycerol.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one week or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<0.5 EU/mg as determined by the LAL method
Amino Acid Sequence
mtdcefgyiy rlaqdylqcv lqipqpgsgp sktsrvlqnv afsvqkevek nlkscldnvn vvsvdtartl fnqvmekefe dgiinwgriv tifafegili kkllrqqiap dvdtykeisy fvaefimnnt gewirqnggw engfvkkfep kshhhhhh

Country of Origin
USA
References
1. Duriez, P. et al. （2000） J. Biol. Chem. 275:18099
2. Simmons, M. J. et al. （2007） Oncogene 27:1421