产品编号:B111-1

产品描述:Recombinant Human BCA-1

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:

说明书:

Recombinant Human B Cell Attracting Chemokine-1 （BCA-1）
Purified No Carrier Protein
（CXCL13, BLC （mouse）, BLR1 Ligand, SCYB13, ANGIE, ANGIE2）
Prod. No.: B111
Source E. coli
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
CXCL13, also known as B-lymphocyte chemoattractant （BLC）, is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein ａnd 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes ａnd a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches ａnd defective formation of primary follicles ａnd germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BCA-1 is Mr 8.7 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 9 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile （CH3CN） ａnd 0.1% trifluoroacetic acid （TFA）.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human B Cell Attracting Chemokine-1 is determined by its ability to chemoattract human B cells using a concentration of 1.0-10.0 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
vlevyyts lrcrcvqess vfiprrfidr iqilprgngc prkeiivwkk nksivcvdpq aewiqrmmev lrkr

Country of Origin
USA
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