产品编号:B131-1
产品描述:Recombinant Human BAFF-R
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human B Cell Activating Factor Receptor （BAFF-R）
Purified No Carrier Protein
（BAFF Receptor BR3, TNFRSF13, BlyS Receptor 3）
Prod. No.: B131
Source E. coli
Pkg. Size: 1 mg, 50 µg
Storage: -20°C Detailed storage instructions below.
Description
Background:
BAFF receptor （BAFF R/TNFRSF13/BlyS） is a type III transmembrane protein ａnd member of the TNF receptor （TNFR） super family. It is highly expressed in spleen, lymph node, resting B cells, peripheral blood leukocytes ａnd to some extent, in activated B cells ａnd resting CD4+ cells. BAFF R plays a role in nearly all stages of differentiation of B cells from development to the maintenance of long-lived bone marrow plasma cells （1）. BAFF R is one of three receptors, including TACI ａnd BCMA, identified for BAFF. BAFF R signals by activation of the NFκB signaling pathway （2）. Overexpression of the BAFF R ligand, BAFF, in mice leads to autoimmune characteristics similar to systemic lupus erythematosus （SLE） ａnd Sjogren’s syndrome. Furthermore, high levels of BAFF have been detected in the serum of humans with SLE, rheumatoid arthritis, Sjogren’s syndrome ａnd systemic sclerosis.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BAFF-R is Mr 34 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 40-50 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes ａnd store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human BAFF Receptor is determined by its ability to block mouse BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 4.0 µg/ml in the presence of 1 µg/ml of human soluble BAFF.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
rrgprslrg rdapaptpcv paecfdllvr hcvacgllrt prpkpagass paprtalqpq esvgagagea adiegrmdpk scdkthtcpp cpapellggp svflfppkpk dtlmisrtpe vtcvvvdvsh edpevkfnwy vdgvevhnak tkpreeqyns tyrvvsvltv lhqdwlngke ykckvsnkal papiektisk akgqprepqv ytlppsrdel tknqvsltcl vkgfypsdia vewesngqpe nnykttppvl dsdgsfflys kltvdksrwq qgnvfscsvm healhnhytq kslslspgk
Country of Origin
USA
References
1. Thompson, J. S. et al. （2001） Science 293:2108
2. Mackay, F. et al. （2002） Nature Rev. Immunol. 2:464
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