产品编号:B115-100

产品描述:Recombinant Human BAFF

反应种属:

实验方法:

标记:

规格:100µg

供应商:Leinco

价格(RMB):9675.00

订购:

说明书:

Recombinant Human B-Cell Activating Factor （BAFF）
Purified No Carrier Protein
（TNFSF13B, TALL-1, BLyS, THANK, zTNF4, BAFF, CD257, TNFSF20, Delta BAFF）
Prod. No.: B115
Source E. coli
Pkg. Size: 1 mg, 5 µg, 20 µg, 100 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
B-cell activating factor （BAFF） is the tumor necrosis factor ligand superfamily member 13B （TNFLSF13B）, also known as B Lymphocyte Stimulator （BLyS） ａnd TNF- ａnd APOL-related leukocyte expressed ligand （TALL-1） ａnd the Dendritic cell-derived TNF-like molecule （CD257 antigen; cluster of differentiation 257）.

BAFF is a glycoprotein which undergoes glycosylation at residue 124. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells ａnd bone marrow stromal cells. The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, ａnd BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes （TACI is also found on a subset of T-cells ａnd BCMA on plasma cells）. TACI binds worst since its affinity is higher for a protein similar to BAFF, called A Proliferation Inducing Ligand （APRIL）. BCMA displays an intermediate binding phenotype ａnd will work with either BAFF or APRIL to varying degrees. Signaling trough BAFF-R ａnd BCMA stimulates B lymphocytes to undergo proliferation ａnd to counter apoptosis. All these ligands act as heterotrimers （i.e. three of the same molecule） interacting with heterotrimeric receptors.

BAFF provides critical survival signals to a subset of B cells with intermediate maturation status （T2 B cells） during the immune response. BAFF also plays an important role in the development of lymphoid tissue ａnd enhances the survival of activated memory B cells. Human ａnd mouse BAFF share 86% aa sequence identity.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human BAFF is Mr 17.0 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 22 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from 10 mM NaP, pH 7.5.
Purity
>90% by SDS-PAGE ａnd HPLC
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein should be aliquoted into working volumes ａnd stored at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human BAFF was assayed using human lyphoblast RPMI 8226 cells ａnd showed a neutralization ED50 of 2 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Country of Origin
USA
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