产品编号:A219-1

产品描述:Recombinant Human Apolipoprotein E4 (ApoE4)

反应种属:

实验方法:

标记:

规格:1mg

供应商:Leinco

价格(RMB):面议

订购:

说明书:

Recombinant Human Apolipoprotein E4 （ApoE4）
Purified No Carrier Protein
Prod. No.: A219
Source E. coli
Pkg. Size: 1 mg, 500 µg
Storage: -20°C Detailed storage instructions below.

Description
Background:
Apolipoprotein E （ApoE） produced by the liver ａnd circulating macrophages, that is a constituent of every plasma lipoprotein except the smallest low density lipoproteins （LDL）. It is a key player in the recycling ａnd redistribution of lipids ａnd cholesterol. ApoE is a ligand with a high affinity for low density lipoprotein receptors （LDLR）. It regulates the activity of enzymes that metabolize lipids ａnd also makes lipids soluble. Mice ａnd humans that lack ApoE cannot remove excess lipoproteins from the plasma ａnd have an increased risk of atherosclerosis. Defective binding of ApoE to its receptors will lead to accumulation of cholesterol-rich lipoprotein particles in the plasma; this is the cause of type III hyperlipoproteinemia. There are three main isoforms of ApoE all products of alleles at a single gene locus: E2 （Cys112, Cys158）, E3 （C112, Arg158）, ａnd E4 （Arg112, Arg158）. Inheritance of ApoE4 has been associated with earlier onset of Alzheimer’s disease, Parkinson’s disease ａnd increased likelihood of dementia.

Source
E. coli
Molecular Weight
The molecular weight of Recombinant Human ApoE4 is Mr 34.4 kDa.
State of Matter
Lyophilized
Formulation
The protein was 0.2 µm filtered ａnd lyophilized from PBS.
Purity
>90% by SDS Page ａnd HPLC
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. For long-term storage of the reconstituted protein add a carrier protein such as 0.1% BSA, aliquot into working volumes ａnd store at -20°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Biological Activity
Data not yet available.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MKVEQAVETE PEPELRQQTE WQSGQRWELA LGRFWDYLRW VQTLSEQVQE ELLSSQVTQE LRALMDETMK ELKAYKSELE EQLTPVAEET RARLSKELQA AQARLGADME DVRGRLVQYR GEVQAMLGQS TEELRVRLAS HLRKLRKRLL RDADDLQKRL AVYQAGAREG AERGLSAIRE RLGPLVEQGR VRAATVGSLA GQPLQERAQA WGERLRARME EMGSRTRDRL DEVKEQVAEV RAKLEEQAQQ IRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKVQAAVGT SAAPVPSDNH

Country of Origin
USA