产品编号:A217-100
产品描述:Recombinant Human Apolipoprotein A-I (ApoA-I)
反应种属:
实验方法:
标记:
规格:100µg
供应商:Leinco
价格(RMB):5400.00
订购:
说明书:
Recombinant Human Apolipoprotein A-I （ApoA-I）
Prod. No.: A217
Source E. coli
Pkg. Size: 1 mg, 100 µg
Description
Background:
Native ApoA-I is the principal protein component of high density lipoprotein （HDL）. ApoA-I gives HDL its ability to transport cholesterol from the periphery to the liver, ａnd thereby prevent cholesterol deposition in the arterial wall. This is why HDL is known as “good cholesterol.” Biochemically, ApoA-I contains an extra cysteine bridge, causing it to exist as a homodimer or as a heterodimer with ApoA-II. However, the enhanced cardioprotective activity of this mutant （which likely depends on cholesterol efflux） cannot easily be replicated by other cysteine mutants.
ApoA-I has recently been shown to be a better indicator of cardiovascular events than low density lipoprotein, revealing the powerful role played by this protein. Availability of the recombinant protein will allow investigators to further elucidate the function of ApoA-I ａnd investigate potential therapeutic applications.
Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, ａnd with systemic non-neuropathic amyloidosis. In one study, a decrease in ApoA1 levels was detected in schizophrenia patients＇ CSF, brain ａnd peripheral tissues.
Source
E. coli
State of Matter
Purity
>97% by SDS-PAGE ａnd HPLC
Storage ａnd Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes ａnd store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
Data not yet available.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ
Country of Origin
USA
Related Products
Native Human ApoA-I （Leinco Prod. No.: A336）
