产品编号:A322-1
产品描述:Recombinant Human Angiopoietin-1（ANG-1）
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Human Angiopoietin-1 （ANG-1）
Purified No Carrier Protein
（AGP1, AGPT, ANGPT1）
Prod. No.: A322
Source NSO Cells
Pkg. Size: 1 mg, 25 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
The angiopoietin （Ang） family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 ａnd Ang-4, activate the Tie2 receptor, whereas Ang-2 ａnd Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 （Ang-1） is a secreted growth factor which binds to ａnd activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival ａnd capillary morphogenesis, ａnd also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor （VEGF） levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin ａnd Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 ａnd mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration ａnd Ang-2 expressed by descending li mbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms＇ kidney tumours ａnd in vascular remodelling after nephrotoxicity. Altogether, existing data suggest that VEGF-A ａnd Angiopoietins not only have quite different roles during vascular development, but also very complementary ａnd coordinated roles.
Human Ang-1 shares approximately 97% ａnd 60% amino acid sequence identity with mouse Ang-1 ａnd human Ang-2, respectively.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Human ANG-1 is Mr 56 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 70 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in Tris-Citrate ａnd sodium chloride （NaCl）.
Purity
>90% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
s nqrrspensg rrynriqhgq caytfilpeh dgncresttd qyntnalqrd aphvepdfss qklqhlehvm enytqwlqkl enyivenmks emaqiqqnav qnhtatmlei gtsllsqtae qtrkltdvet qvlnqtsrle iqllenslst yklekqllqq tneilkihek nsllehkile megkhkeeld tlkeekenlq glvtrqtyii qelekqlnra ttnnsvlqkq qlelmdtvhn lvnlctkegv llkggkreee kpfrdcadvy qagfnksgiy tiyinnmpep kkvfcnmdvn gggwtviqhr edgsldfqrg wkeykmgfgn psgeywlgne fifaitsqrq ymlrielmdw egnraysqyd rfhignekqn yrlylkghtg tagkqsslil hgadfstkda dndncmckca lmltggwwfd acgpsnlngm fytagqnhgk lngikwhyfk gpsyslrstt mmirpldfhh hhhh
Country of Origin
USA
References
1. （2006 Dec.）Curr. Mol. Med. 6（8）:831
2. （2006 Sep.） Exp. Clin. Endocrinol. Diabetes 114（8）:438
3. （2006 Oct.） Physiol. Genomics 3;27（1）:20
4. （2006 Sep.） Microcirculation 13（6）:423
5. （2006） Reprod. Fertil. Dev. 18（5）:509
6. （2006 Jun.） Cancer Res. 15;66（12）:6167
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