产品编号:A339-250

产品描述:Recombinant Human Angiogenin

反应种属:

实验方法:

标记:

规格:250µg

供应商:Leinco

价格(RMB):14850.00

订购:

说明书:

Recombinant Human Angiogenin （ANG）
Purified No Carrier Protein
（ALS9, HEL168, RNASE4, RNASE5, MGC22466, MGC71966）
Prod. No.: A339
Source E. coli
Pkg. Size: 1 mg, 50 µg, 250 µg
Storage: -20°C to -70°C Detailed storage instructions below.

Description
Background:
Angiogenin （Ang）, also known as ALS9, is a member of the ribonuclease superfamily. It exhibits both angiogenic ａnd non-angiogenic activities ａnd is involved in endothelial cell migration, proliferation ａnd differentiation. Ang shares approximately 35% amino acid sequence identity with pancreatic RNase. Although Ang has the same general catalytic properties as RNase A, its activity differs markedly both in magnitude ａnd in specificity （1）. The enzymatic activity of Ang appears to be essential for biological activity: replacements of important active site residues invariably diminish ribonuclease ａnd angiogenesis activities in parallel, ａnd a substitution that increases enzymatic activity also enhances angiogenic potency （2）. Like other members of the ribonuclease superfamily, Ang is a cytotoxic agent that can eliminate cellular protein synthesis. It causes inhibition of protein synthesis by functioning as a cytotoxic tRNA-specific RNase （3）. Ang stimulates capillary ａnd umbilical vein endothelial cells to produce diacylglycerol ａnd secrete prostacyclin by phospholipase activation （4）. It is produced by a variety of tumor ａnd normal cell types. Cells that express Ang include vascular endothelial ａnd smooth muscle cells, fibroblasts, normal colonic epithelium, normal peripheral blood lymphocytes, lung ａnd colonic epithelial tumor cell lines, ａnd primary gastrointestinal adenocarcinomas. Ang is also present in normal human plasma. Research findings suggest that Ang also has antimicrobial properties （5）. Researchers have identified several Ang mutations that may increase the risk of developing amyotrophic lateral sclerosis （ALS） （6）.

Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human ANG is Mr 14 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 14 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>97% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Amino Acid Sequence
qdnsry thfltqhyda kpqgrddryc esimrrrglt spckdintfi hgnkrsikai cenkngnphr enlriskssf qvttcklhgg spwppcqyra tagfrnvvva cenglpvhld qsifrrp

Country of Origin
USA
References
1. Shapiro, R. et al. （1986） Biochemistry 25:3527
2. Shapiro, R. et al. （1990） Biochemistry 28:7401
3. Saxena, S. K. et al. （1992） J. Biol. Chem. 267:21982
4. Bicknell, R. et al. （1989） Proc. Natl. Acad. Sci. （USA） 86:1573
5. Ganz, T. et al. （2003） Nature Immunol. 4:213
6. Subramanian, V. et al. （2007） Hum. Mol. Gen. 17:130

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