产品编号:A349-1
产品描述:Recombinant Mouse ALK-1
反应种属:
实验方法:
标记:
规格:1mg
供应商:Leinco
价格(RMB):面议
订购:
说明书:
Recombinant Mouse Activin Receptor Like Kinase-1 （ALK-1）
Purified No Carrier Protein
（ACVRL1, ACVRLK1, HHT, HHT2, ORW2, SKR3, TSR-I）
Prod. No.: A349
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Activin A receptor type II-like 1, also known as ACVRL1, is a human gene. ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as "activin receptor-like kinase 1", or "ALK1". This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- ａnd serine-rich region （called the GS domain） preceding the kinase domain, ａnd a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 suggesting a critical role for ALK-1 in the control of blood vessel development or repair.
Human ａnd mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of Recombinant Mouse ALK-1 is Mr 37.7 kDa （monomer）. However, the actual molecular weight as observed by migration on SDS Page is Mr 50-55 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered ａnd lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE ａnd analyzed by silver stain.
Storage ａnd Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
Measured by its ability to inhibit recombinant Human BMP-9 induced alkaline phosphatase production by ATDC5 cells （David, L., et al., 2007, Blood, 109:1953 - 61）. The ED50 for this effect is 20 - 80 ng/ml in the presence of 2 ng/ml of recombinant Human BMP-9.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
dlrrpskl vnctcesphc krpfcqgswc tvvlvreqgr hpqvyrgcgs lnqelclgrp teflnhhccy rsfcnhnvsl mleatqtpse epevdahlpi egrmdpkscd kthtcppcpa pellggpsvf lfppkpkdtl misrtpevtc vvvdvshedp evkfnwyvdg vevhnaktkp reeqynstyr vvsvltvlhq dwlngkeykc kvsnkalpap iektiskakg qprepqvytl ppsrdeltkn qvsltclvkg fypsdiavew esngqpenny kttppvldsd gsfflysklt vdksrwqqgn vfscsvmhea lhnhytqksl slspgk
Country of Origin
USA
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