产品编号:F156-100
产品描述:Recombinant Human 4-1BB Receptor
反应种属:
实验方法:
标记:
规格:100µg
供应商:Leinco
价格(RMB):4875.00
订购:
说明书:
Recombinant Human 4-1BB Receptor (4-1BBR)
Purified No Carrier Protein
(TNFRSF9, CD137 Antigen, T-Cell Antigen ILA)
Prod. No.: F156
Source NSO Cells
Pkg. Size: 1 mg, 100 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
4-1BB Receptor, a member of the TNF superfamily of receptors, is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB Receptor binds to 4-1BBL to provide a co-stimulatory signal for T lymphocytes. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells.
Source
NSO Cells
Molecular Weight
The predicted molecular weight of the reduced Human 4-1BBR monomer is Mr 44.8 kDa (monomer). However, the actual molecular weight, due to glycosylation, as observed by migration on SDS Page is Mr 50-65 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human 4-1BB Receptor is determined by its inhibitory effect of the 4-1BBL mediated stimulation of IL-8 production by human PBMC. About 90% of inhibition was seen using a concentration of 1 µg for both 4-1BBL and 4-1BBR.
The activity of the receptor was also measured by its ability to bind Recombinant Human 4-1BB Ligand in a functional ELISA. A coating concentration of 1 µg/ml was used for this assay.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
lqdpcsn cpagtfcdnn rnqicspcpp nsfssaggqr tcdicrqckg vfrtrkecss tsnaecdctp gfhclgagcs mceqdckqgq eltkkgckdc cfgtfndqkr gicrpwtncs ldgksvlvng tkerdvvcgp spadlspgas svtppapare pghspqdieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh
Country of Origin
USA
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