产品编号:C294
产品描述:Anti-Human CDw60 (9-0-acetyl GD3)-R-phycoerythrin (R-PE)
反应种属:
实验方法:
标记:
规格:100ug
供应商:Leinco
价格(RMB):3300.00
说明书:
Anti-Human CDw60
R-phycoerythrin (R-PE)
(9-0-acetyl GD3)
Monoclonal Antibody
Prod. No.: C294
Clone: Jones
Isotype: Mouse IgM
Conc.: 0.1 mg/ml
Pkg. Size: 50 µg, 100 µg, 200 µg
Description
Specificity:
Mouse Anti-Human CDw60 (Clone Jones) recognizes Human CDw60. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CDw60 recognizes a (Mr 120 kD) carbohydrate (9-0-acetyl GD3) antigen.
Antigen Distribution:
The CDw60 antigen is present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells. The antigen is also detectable in some previously undescribed benign and malignant tumors. CDw60 shows different subcellular distribution patterns.
Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Host Species
Mouse
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (Frozen), FC
FC: This antibody can be used at a concentration of ≤1 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
References
1. Leukocyte Typing V (1995) Oxford University Press
2. Blum, A. S. and Barnstable, C. J. et al. (1987) Proc. Natl. Acad. Sci. 84:8716
3. Constantine-Paton, M. et al. (1986) Nature 324:659
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.